Sequencing of FKS Hot Spot 1 from Saprochaete capitata To Search for a Relationship to Reduced Echinocandin Susceptibility.
Antimicrob Agents Chemother
; 62(2)2018 02.
Article
de En
| MEDLINE
| ID: mdl-29229638
ABSTRACT
Saprochaete capitata, formerly known as Geotrichum capitatum, is an emerging fungal pathogen with low susceptibility to echinocandins. Here, we report the nucleotide sequence of the S. capitata hot spot 1 region of the FKS gene (FKS HS1), which codifies for the catalytic subunit of ß-1,3-d-glucan synthase, the target of echinocandins. For that purpose, we first designed degenerated oligonucleotide primers derived from conserved flanking regions of the FKS1 HS1 segment of 12 different fungal species. Interestingly, analysis of the translated FKS HS1 sequences of 12 isolates of S. capitata revealed that all of them exhibited the same F-to-L substitution in a position that is highly related to reduced echinocandin susceptibility.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines fongiques
/
Régulation de l'expression des gènes fongiques
/
Résistance des champignons aux médicaments
/
Échinocandines
/
Geotrichum
/
Glucosyltransferases
/
Antifongiques
Limites:
Humans
Langue:
En
Journal:
Antimicrob Agents Chemother
Année:
2018
Type de document:
Article
Pays d'affiliation:
Espagne