Fish DNA barcoding around large marine infrastructure for improved biodiversity assessment and monitoring.
Mitochondrial DNA A DNA Mapp Seq Anal
; 29(8): 1174-1179, 2018 12.
Article
de En
| MEDLINE
| ID: mdl-29373939
ABSTRACT
Accurate species-level identification is pivotal for environmental assessments and monitoring. The PERU LNG terminal is composed of large marine infrastructure located on the central coast of Peru. Since construction, taxonomically challenging species such as drum fishes (Sciaenidae) have been attracted to the new hard-bottom habitat. We conducted a DNA barcoding study to investigate fish diversity and constructed a DNA barcode reference library. We examined 56 vouchered specimens and identified 24 unique species. Intra- and interspecific divergence estimates ranged between 0 and 0.64% and 11 and 35.5%, respectively. We assessed the efficiency of the reference library to identify 29 non-vouchered specimens. We had 82.5% efficiency by using both our reference library (n = 17) and GenBank (n = 24). We highlight the importance of implementing molecular barcoding for complementing biodiversity assessments in marine environments. This study represents a first step towards generating a comprehensive DNA barcode reference library for marine fishes in Peru.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Perciformes
/
Biodiversité
/
Codage à barres de l'ADN pour la taxonomie
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Mitochondrial DNA A DNA Mapp Seq Anal
Année:
2018
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique