[Effect investigation of coumarin constituents in Angelica dahurica on pharmacokinetics of docetaxel by LC-MS].
Zhongguo Zhong Yao Za Zhi
; 42(24): 4870-4874, 2017 Dec.
Article
de Zh
| MEDLINE
| ID: mdl-29493160
ABSTRACT
The study was aimed to establish a liquid chromatography-tandem mass spectrometric method for the determination of the docetaxel concentration in rat plasma, and study the effect of coumarin constituents (imperatorin, isoimperatorin and oxypeucedanin) in Angelica dahurica on pharmacokinetics of docetaxel.Plasma was precipitated with acetonitrile and determined by LC-MS method with Paclitaxel as an internal standard. The specificity, linearity, range, accuracy, precision and stability of the method were suitable for the determination of docetaxel in plasma.Six sprague-dawley rats in each group received intragastric administration of docetaxel (50 mgâ¢kg⻹), oxypeucedanin (8 mgâ¢kg⻹) combined with docetaxel (50 mgâ¢kg⻹), imperatorin (15 mgâ¢kg⻹) combined with docetaxel (50 mgâ¢kg⻹), and isoimperatorin(15 mgâ¢kg⻹) combined with docetaxel (50 mgâ¢kg⻹).Their drug plasma concentration was determined by LC-MS with Paclitaxel as an internal standard to draw plasma concentration-time curve, and the phamacokinetic parameters were calculated by DAS 2.0. The results showed that the phamacokinetic parameters of docetaxel all had notable changes when combined with imperatorin, isoimperatorin, and oxypeucedanin, respectively. The phamacokinetic parameters AUC and Cmax were significantly increased, indicating that coumarin constituents in Angelica dahurica could promote the oral bioavailability of docetaxel, and their effects were in the following order oxypeucedanin> isoimperatorin> imperatorin.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Furocoumarines
/
Angelica
/
Docetaxel
Limites:
Animals
Langue:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Sujet du journal:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Année:
2017
Type de document:
Article
Pays d'affiliation:
Chine