Puerarin promotes MIN6 cell survival by reducing cellular reactive oxygen species.
Mol Med Rep
; 17(5): 7281-7286, 2018 05.
Article
de En
| MEDLINE
| ID: mdl-29568901
ABSTRACT
Type 1 diabetes is caused by destruction of the pancreatic ßcells and, to date, no cure has been developed. Promoting the survival of pancreatic ßcells may be beneficial for patients with type 1 diabetes. Puerarin is an estrogen analogue that been demonstrated in previous studies to be able to decreased blood glucose in patients with type 1 diabetes. Similar results were demonstrated in previous studies which additionally demonstrated that puerarin was able to decreased blood glucose in type 1 diabetic mice by protecting pancreatic ßcells. However, the mechanism underlying the function of puerarin in pancreatic ßcells remains unclear. Therefore, the present study sought to investigate the detailed function of puerarin in pancreatic ßcells. In the present study, H2O2 was used to induce apoptosis. It was observed that puerarin significantly decreased H2O2induced apoptosis in mouse insulinoma MIN6 cells. It was additionally observed that puerarin decreased the levels of intracellular reactive oxygen species and mitochondrial superoxide in MIN6 cells. The protective effect of puerarin was markedly decreased by 6aminonicotinamide, an inhibitor of glucose6phosphate dehydrogenase (G6PD). In conclusion, the results of the present study suggested that puerarin may increase the activity of G6PD, decreased the level of oxidative stress in MIN6 cells, protect mitochondria and promote MIN6 cell survival. Investigating the mechanism underlying the effect of puerarin in MIN6 cells may provide a novel approach for development of a cure for type 1 diabetes.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Espèces réactives de l'oxygène
/
Apoptose
/
Stress oxydatif
/
Cellules à insuline
/
Isoflavones
/
Antioxydants
Limites:
Animals
Langue:
En
Journal:
Mol Med Rep
Année:
2018
Type de document:
Article