Modulation of Adipocyte Differentiation and Proadipogenic Gene Expression by Sulforaphane, Genistein, and Docosahexaenoic Acid as a First Step to Counteract Obesity.
Oxid Med Cell Longev
; 2018: 1617202, 2018.
Article
de En
| MEDLINE
| ID: mdl-29576843
ABSTRACT
Obesity is characterized by excess body fat accumulation due to an increase in the size and number of differentiated mature adipocytes. Adipocyte differentiation is regulated by genetic and environmental factors, and its inhibition could represent a strategy for obesity prevention and treatment. The current study was designed with two aims:
(i) to evaluate the changes in the expression of adipogenic markers (C/EBPα, PPARγ variant 1 and variant 2, and GLUT4) in 3T3-L1 murine preadipocytes at four stages of the differentiation process and (ii) to compare the effectiveness of sulforaphane, genistein, and docosahexaenoic acid in reducing lipid accumulation and modulating C/EBPα, PPARγ1, PPARγ2, and GLUT4 mRNA expression in mature adipocytes. All bioactive compounds were shown to suppress adipocyte differentiation, although with different effectiveness. These results set the stage for further studies considering natural food constituents as important agents in preventing or treating obesity.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acide docosahexaénoïque
/
Isothiocyanates
/
Adipocytes
/
Génistéine
/
Adipogenèse
/
Obésité
Limites:
Animals
Langue:
En
Journal:
Oxid Med Cell Longev
Sujet du journal:
METABOLISMO
Année:
2018
Type de document:
Article
Pays d'affiliation:
Italie