A rabbit model of corneal Ectasia generated by treatment with collagenase type II.
BMC Ophthalmol
; 18(1): 94, 2018 Apr 13.
Article
de En
| MEDLINE
| ID: mdl-29653563
ABSTRACT
BACKGROUND:
To investigate use of collagenase type II for generating a rabbit model of corneal ectasia.METHODS:
Ten New Zealand white rabbits were used with right eyes treated as the experimental group and left eyes treated as the control group. After epithelial debridement, a collagenase type II solution (200 µL of 5 mg/mL) was applied in the experimental group at room temperature (24 °C) for 30 min, and a 200 µL solution without collagenase was applied in the control group. Slit-lamp microscopy, the mean keratometry (Km), and central cornea thickness (CCT) were examined before and after the procedure. Corneas were obtained on day 14 for biomechanical evaluation.RESULTS:
No obvious inflammatory reaction was observed in all eyes after the procedure. A statistically significant increase in Km (1.54 ± 1.29D vs - 0.82 ± 0.44D at day7 and 0.89 ± 0.89D vs - 2.11 ± 1.02D at day14) and a statistically significant decrease in CCT (- 23.10 ± 12.17 µm vs 6.20 ± 16.51 µm at day7 and - 16.10 ± 10.46 µm vs 11.60 ± 0.88 µm at day14) were observed in the experimental group compared with the control group. The mean stresses and elastic modulus at 5%, 10%, 15%, and 20% deformities in the experimental group decreased and the differences in elastic modulus between the two groups were statistically significant at 10% and 15% deformities.CONCLUSIONS:
Collagenase type II treatment results in mimic KC with increased corneal keratometry and corneal thinning and a lower elastic modulus. An animal model for corneal ectasia can be generated by treatment with collagenase type II.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Collagenases
/
Cornée
/
Kératocône
Limites:
Animals
Langue:
En
Journal:
BMC Ophthalmol
Sujet du journal:
OFTALMOLOGIA
Année:
2018
Type de document:
Article
Pays d'affiliation:
Chine