Your browser doesn't support javascript.
loading
Nanoparticle Wettability Influences Nanoparticle-Phospholipid Interactions.
Konduru, Nagarjun V; Damiani, Flavia; Stoilova-McPhie, Svetla; Tresback, Jason S; Pyrgiotakis, Georgios; Donaghey, Thomas C; Demokritou, Philip; Brain, Joseph D; Molina, Ramon M.
Affiliation
  • Stoilova-McPhie S; Center for Nanoscale Systems, Faculty of Art and Sciences , Harvard University , 11 Oxford Street , Cambridge , Massachusetts 02138 , United States.
  • Tresback JS; Center for Nanoscale Systems, Faculty of Art and Sciences , Harvard University , 11 Oxford Street , Cambridge , Massachusetts 02138 , United States.
Langmuir ; 34(22): 6454-6461, 2018 06 05.
Article de En | MEDLINE | ID: mdl-29754486
ABSTRACT
We explored the influence of nanoparticle (NP) surface charge and hydrophobicity on NP-biomolecule interactions by measuring the composition of adsorbed phospholipids on four NPs, namely, positively charged CeO2 and ZnO and negatively charged BaSO4 and silica-coated CeO2, after exposure to bronchoalveolar lavage fluid (BALf) obtained from rats, and to a mixture of neutral dipalmitoyl phosphatidylcholine (DPPC) and negatively charged dipalmitoyl phosphatidic acid (DPPA). The resulting NP-lipid interactions were examined by cryogenic transmission electron microscopy (cryo-TEM) and atomic force microscopy (AFM). Our data show that the amount of adsorbed lipids on NPs after incubation in BALf and the DPPC/DPPA mixture was higher in CeO2 than in the other NPs, qualitatively consistent with their relative hydrophobicity. The relative concentrations of specific adsorbed phospholipids on NP surfaces were different from their relative concentrations in the BALf. Sphingomyelin was not detected in the extracted lipids from the NPs despite its >20% concentration in the BALf. AFM showed that the more hydrophobic CeO2 NPs tended to be located inside lipid vesicles, whereas less hydrophobic BaSO4 NPs appeared to be outside. In addition, cryo-TEM analysis showed that CeO2 NPs were associated with the formation of multilamellar lipid bilayers, whereas BaSO4 NPs with unilamellar lipid bilayers. These data suggest that the NP surface hydrophobicity predominantly controls the amounts and types of lipids adsorbed, as well as the nature of their interaction with phospholipids.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Phospholipides / Mouillabilité / Nanoparticules Limites: Animals Langue: En Journal: Langmuir Sujet du journal: QUIMICA Année: 2018 Type de document: Article

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Phospholipides / Mouillabilité / Nanoparticules Limites: Animals Langue: En Journal: Langmuir Sujet du journal: QUIMICA Année: 2018 Type de document: Article