Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos.
Methods Cell Biol
; 144: 431-440, 2018.
Article
de En
| MEDLINE
| ID: mdl-29804681
ABSTRACT
Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple using various microinjection setups, metaphase-II mouse eggs are notoriously fragile, and nondamaging microinjection can be difficult to achieve. Here we describe a microinjection method that is based on electrophysiology, which vastly reduces microinjection damage, especially in metaphase-II eggs. When optimized, this approach allows for over 90% oocyte survival, increasing confidence in experimental results.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ovocytes
/
Division cellulaire
/
Électricité
/
Embryon de mammifère
/
Fécondation
/
Mammifères
/
Microinjections
Limites:
Animals
Langue:
En
Journal:
Methods Cell Biol
Année:
2018
Type de document:
Article