SChitosan-capped gold nanoparticles impair radioresistant glioblastoma stem-like cells.
J BUON
; 23(3): 800-813, 2018.
Article
de En
| MEDLINE
| ID: mdl-30003755
ABSTRACT
PURPOSE:
Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresistant glioblastoma stem cells (GSCs). Herein, we tested if chitosan-capped gold nanoparticles (Chit-GNPs) may overcome the limitations of drug concentrations by increased cell internalization in GSCs and if such GNPs could enhance the response to irradiation.METHODS:
Chitosan was used for Chit-GNP synthesis as a reducing and stabilizing agent. Chit-GNPs were characterized by spectroscopy, dark field, transmission electron microscopy and zeta potential measurements. Patient-derived GSCs and human osteoblasts were treated with increasing concentrations of nanoparticles and irradiated. The uptake and cytotoxicity of Chit-GNPs were compared to that of uncoated GNPs.RESULTS:
The positively-charged, 26 nm-sized, spherical Chit-GNPs, showed a huge intracellular accumulation into the cytosol, lysosomes and near the nucleus, whereas no uncoated GNPs were internalized within GSCs. Surprisingly, Chit-GNPs were highly cytotoxic for GSCs irrespective of cell irradiation, that failed to add an additional benefit when combined with Chit-GNPs/GNPs. Moreover, Chit-GNPs were selectively cytotoxic for GSCs and did not affect the normal cells, despite an increased nanoparticle internalization.CONCLUSIONS:
The important Chit-GNP internalization and their selective cytotoxicity for GSCs make this compound a potential novel anticancer agent and a promising backbone for drug delivery in glioblastoma.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules souches
/
Glioblastome
/
Chitosane
/
Nanoparticules métalliques
/
Or
Limites:
Humans
Langue:
En
Journal:
J BUON
Sujet du journal:
NEOPLASIAS
Année:
2018
Type de document:
Article
Pays d'affiliation:
Roumanie
Pays de publication:
CHIPRE
/
CY
/
CYPRUS