Inhibition of microRNA-505 suppressed MPP+â¯-induced cytotoxicity of SHSY5Y cells in an in vitro Parkinson's disease model.
Eur J Pharmacol
; 835: 11-18, 2018 Sep 15.
Article
de En
| MEDLINE
| ID: mdl-30025813
ABSTRACT
Parkinson's disease (PD) is a progressive and incurable neurodegenerative disorder. In this study, we utilized SHSY5Y cells an in vitro PD model to study the biological function of microRNA-505 (miR-505) in 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity. SHSY5Y cells were treated with MPP+â¯to induce PD-associated cell death and cytotoxicity. MiR-505 was found to be upregulated by MPP+â¯. Lentiviral transduction mediated miR-505 downregulation protected SHSY5Y cells from MPP+â¯-induced cell death, whereas miR-505 upregulation had no effect on MPP+â¯-induced SHSY5Y cell death. MiR-505 downregulation also ameliorated MPP+â¯-induced cytotoxicity, by preserving MMP and reducing LDH level, caspase-3 and reactive oxygen species activities. Possible downstream target of miR-505, neural proliferation, differentiation and control 1 (NPDC1) was confirmed to be the downstream target of miR-505 in SHSY5Y cells. In addition, NPDC1 knockdown was shown to reverse the protection of miR-505-downregulation on MPP+â¯-induced cytotoxicity. Thus, our study demonstrated that miR-505, by inversely regulating NPDC1 may have a functional role in regulating human Parkinson's disease.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Maladie de Parkinson
/
1-Méthyl-4-phényl-pyridinium
/
MicroARN
/
Techniques de knock-down de gènes
Limites:
Humans
Langue:
En
Journal:
Eur J Pharmacol
Année:
2018
Type de document:
Article