Mechanotransduction in talin through the interaction of the R8 domain with DLC1.
PLoS Biol
; 16(7): e2005599, 2018 07.
Article
de En
| MEDLINE
| ID: mdl-30028837
ABSTRACT
The mechanical unfolding of proteins is a cellular mechanism for force transduction with potentially broad implications in cell fate. Despite this, the mechanism by which protein unfolding elicits differential downstream signalling pathways remains poorly understood. Here, we used protein engineering, atomic force microscopy, and biophysical tools to delineate how protein unfolding controls cell mechanics. Deleted in liver cancer 1 (DLC1) is a negative regulator of Ras homolog family member A (RhoA) and cell contractility that regulates cell behaviour when localised to focal adhesions bound to folded talin. Using a talin mutant resistant to force-induced unfolding of R8 domain, we show that talin unfolding determines DLC1 downstream signalling and, consequently, cell mechanics. We propose that this new mechanism of mechanotransduction may have implications for a wide variety of associated cellular processes.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Taline
/
Protéines d'activation de la GTPase
/
Protéines suppresseurs de tumeurs
/
Mécanotransduction cellulaire
Limites:
Animals
Langue:
En
Journal:
PLoS Biol
Sujet du journal:
BIOLOGIA
Année:
2018
Type de document:
Article
Pays d'affiliation:
Royaume-Uni