Durable targeting of B-lymphocytes in living mice.
Sci Rep
; 8(1): 11143, 2018 07 24.
Article
de En
| MEDLINE
| ID: mdl-30042489
ABSTRACT
Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Transduction génétique
/
Lymphocytes B
/
Antigènes CD19
/
Anticorps monoclonaux d'origine murine
Limites:
Animals
Langue:
En
Journal:
Sci Rep
Année:
2018
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique