In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display.
Methods Mol Biol
; 1827: 269-285, 2018.
Article
de En
| MEDLINE
| ID: mdl-30196502
ABSTRACT
Single-domain antibody (e.g., Nanobody, VHH antibody) is a promising scaffold for therapeutic and diagnostic reagents. To expand the range of target molecules, in vitro selection using cell-free display technologies such as cDNA display is useful and powerful because of their huge libraries and robust stability. We provide technical details for in vitro selection of single-domain antibodies using cDNA display.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ADN complémentaire
/
Anticorps à domaine unique
/
Techniques d'exposition à la surface cellulaire
Limites:
Animals
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2018
Type de document:
Article
Pays d'affiliation:
Japon