Drebrin 1 in dendritic cells regulates phagocytosis and cell surface receptor expression through recycling for efficient antigen presentation.
Immunology
; 156(2): 136-146, 2019 02.
Article
de En
| MEDLINE
| ID: mdl-30317558
ABSTRACT
Phagocytosis, macropinocytosis and antigen presentation by dendritic cells (DC) requires reorganization of the actin cytoskeleton. Drebrin (Dbn1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. However, its role in DC phagocytosis and antigen presentation is unknown. These studies now report that silencing of Dbn1 in DC resulted in restrained cell surface display of receptors, most notably MHC class I and II and co-stimulatory molecules. This, as expected, resulted in impaired antigen-specific T-cell activation and proliferation. Studies additionally revealed that knockdown of Dbn1 in DC impaired macropinocytosis and phagocytosis. However, there was a concomitant increase in fluid-phase uptake, suggesting that Dbn1 is responsible for the differential control of macropinocytosis versus micropinocytosis activities. Taken together, these findings now reveal that Dbn1 plays a major role in coordinating the actin cytoskeletal activities responsible for antigen presentation in DC.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phagocytose
/
Cellules dendritiques
/
Neuropeptides
/
Antigènes d'histocompatibilité de classe I
/
Antigènes d'histocompatibilité de classe II
/
Régulation de l'expression des gènes
/
Présentation d'antigène
Limites:
Animals
Langue:
En
Journal:
Immunology
Année:
2019
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique