Gene Disruption Using CRISPR-Cas9 Technology.
Methods Mol Biol
; 1881: 201-209, 2019.
Article
de En
| MEDLINE
| ID: mdl-30350208
ABSTRACT
The emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology provides tools for researchers to modify genomes in a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to analyzing the desired gene disruption events.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ciblage de gène
/
Systèmes CRISPR-Cas
/
Édition de gène
Limites:
Humans
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2019
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique