Quantitative characterization of single-cell adhesion properties by atomic force microscopy using protein-functionalized microbeads.
J Mol Recognit
; 32(3): e2767, 2019 03.
Article
de En
| MEDLINE
| ID: mdl-30403313
ABSTRACT
A method was developed to characterize the adhesion properties of single cells by using protein-functionalized atomic force microscopy (AFM) probes. The quantification by force spectroscopy of the mean detachment force between cells and a gelatin-functionalized colloidal tip reveals differences in cell adhesion properties that are not within reach of a traditional bulk technique, the washing assay. In this latter method, experiments yield semiquantitative and average adhesion properties of a large population of cells. They are also limited to stringent conditions and cannot highlight disparities in adhesion in the subset of adherent cells. In contrast, this AFM-based method allows for a reproducible and quantitative investigation of the adhesive properties of individual cells in common cell culture conditions and allows for the detection of adhesive subpopulations of cells. These characteristics meet the critical requirements of many fields, such as the study of cancer cell migratory abilities.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de la matrice extracellulaire
/
Analyse sur cellule unique
/
Gélatine
Limites:
Humans
Langue:
En
Journal:
J Mol Recognit
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2019
Type de document:
Article
Pays d'affiliation:
France