Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans.
Bio Protoc
; 8(20)2018 Oct 20.
Article
de En
| MEDLINE
| ID: mdl-30467549
ABSTRACT
C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Bio Protoc
Année:
2018
Type de document:
Article
Pays d'affiliation:
Espagne