Phosphatidylcholine could protect the defect of zearalenone exposure on follicular development and oocyte maturation.
Aging (Albany NY)
; 10(11): 3486-3506, 2018 11 25.
Article
de En
| MEDLINE
| ID: mdl-30472698
ABSTRACT
Zearalenone (ZEA) is a well-known exogenous endocrine disruptor and can lead to severe negative effects on the human and animal reproductive process. Using a follicle culture model, we have previously shown that ZEA exposure significantly affected the follicular development and antrum formation but the underlying mechanisms are not well known. Therefore, in this study, we explored the metabolomic changes of granulosa cell (GC) culture media with or without ZEA exposure. The results showed that ZEA significantly increased phosphatidylcholine or phosphatidyl ethanolamine adducts in culture medium. A comprehensive analysis with the metabolome data from follicular fluid of small and large antral follicles showed that lyso phosphatidylcholine (LPC) was accumulated during follicle growth, but was depleted by ZEA exposure. Exogenous supplement with LPC to the follicle growth media or oocyte maturation media can partly protect the defect of ZEA exposure on follicular antrum formation and oocyte maturation. Taken together, our results demonstrate that ZEA exposure hinders the follicular growth and exogenous LPC can practically protect the defect of ZEA on follicular development and oocyte maturation.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ovocytes
/
Phosphatidylcholines
/
Zéaralénone
/
Follicule ovarique
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Aging (Albany NY)
Sujet du journal:
GERIATRIA
Année:
2018
Type de document:
Article
Pays d'affiliation:
Chine