Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices.
Macromol Biosci
; 19(2): e1800389, 2019 02.
Article
de En
| MEDLINE
| ID: mdl-30511815
ABSTRACT
A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due to the transplantation procedures involving injections of cell suspensions that are associated with cell death, uneven cell distribution, and cell washout. Encapsulating cells in protective hydrogel matrices offers a tissue engineering solution to safely achieve 3D growth of viable transplant cells for implantation into injury sites, to improve regenerative outcomes. It is shown for the first time that canine OMCs (cOMCs) can be propagated with high viability in 3D collagen matrices. Further, a method to incorporate cOMCs pre-labeled with clinical-grade iron oxide nanoparticles into the constructs is described. Intraconstruct labeled cells are visualized using magnetic resonance imaging, offering substantial promise for in vivo tracking of cOMCs delivered in protective matrices.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Traumatismes de la moelle épinière
/
Oligodendroglie
/
Hydrogels
/
Ingénierie tissulaire
/
Cellules souches neurales
Limites:
Animals
Langue:
En
Journal:
Macromol Biosci
Sujet du journal:
BIOQUIMICA
Année:
2019
Type de document:
Article
Pays d'affiliation:
Royaume-Uni