In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector.
Methods Mol Biol
; 1895: 135-147, 2019.
Article
de En
| MEDLINE
| ID: mdl-30539535
ABSTRACT
When using bicistronic expression constructs the issue arises concerning proper evaluation of the cytotoxic efficiency of a combination of therapeutic genes. For this purpose, an approach can be applied based on the transient transfection of cultured human cells with a specifically designed set of mono- and bicistronic expression constructs and on the comparison of their cytotoxic effects. Here the application of this approach is described using an example of the evaluation of the combined cytotoxic action of bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion protein (FCU1) and hepatitis A virus 3C protease in a bicistronic plasmid construct.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pentosyltransferases
/
Protéines virales
/
Cysteine endopeptidases
/
Thérapie génétique
/
Cytosine deaminase
/
Gènes-suicide transgéniques
/
Vecteurs génétiques
Limites:
Humans
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2019
Type de document:
Article
Pays d'affiliation:
Russie