Transcriptome profiling of Plasmodium vivax in Saimiri monkeys identifies potential ligands for invasion.
Proc Natl Acad Sci U S A
; 116(14): 7053-7061, 2019 04 02.
Article
de En
| MEDLINE
| ID: mdl-30872477
Unlike the case in Asia and Latin America, Plasmodium vivax infections are rare in sub-Saharan Africa due to the absence of the Duffy blood group antigen (Duffy antigen), the only known erythrocyte receptor for the P. vivax merozoite invasion ligand, Duffy binding protein 1 (DBP1). However, P. vivax infections have been documented in Duffy-negative individuals throughout Africa, suggesting that P. vivax may use ligands other than DBP1 to invade Duffy-negative erythrocytes through other receptors. To identify potential P. vivax ligands, we compared parasite gene expression in Saimiri and Aotus monkey erythrocytes infected with P. vivax Salvador I (Sal I). DBP1 binds Aotus but does not bind to Saimiri erythrocytes; thus, P. vivax Sal I must invade Saimiri erythrocytes independent of DBP1. Comparing RNA sequencing (RNAseq) data for late-stage infections in Saimiri and Aotus erythrocytes when invasion ligands are expressed, we identified genes that belong to tryptophan-rich antigen and merozoite surface protein 3 (MSP3) families that were more abundantly expressed in Saimiri infections compared with Aotus infections. These genes may encode potential ligands responsible for P. vivax infections of Duffy-negative Africans.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmodium vivax
/
Protéines de protozoaire
/
Paludisme à Plasmodium vivax
/
Récepteurs de surface cellulaire
/
Analyse de profil d'expression de gènes
/
Système Duffy
/
Érythrocytes
/
Antigènes de protozoaire
Limites:
Animals
Langue:
En
Journal:
Proc Natl Acad Sci U S A
Année:
2019
Type de document:
Article
Pays de publication:
États-Unis d'Amérique