On-Site MinION Sequencing.
Adv Exp Med Biol
; 1129: 143-150, 2019.
Article
de En
| MEDLINE
| ID: mdl-30968366
DNA sequencing has reached an unprecedented level with the advent of Oxford Nanopore Technologies' MinION. The low equipment investment, ease of library preparation, small size, and powered only by a laptop computer enable the portability for on-site sequencing. MinION has had its role in clinical, biosecurity, and environmental fields. Here, we describe the many facets of on-site sequencing with MinION. First, we will present some field works using MinION. We will discuss the requirements for targeted or whole genome sequencing and the challenges faced by each technique. We will also elaborate the bioinformatics procedures available for data analysis in the field. MinION has greatly changed the way we do sequencing by bringing the sequencer closer to the biodiversity. Although numerous limitations exist for MinION to be truly portable, improvements of procedures and equipment will enhance MinION's role in the field.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Séquençage nucléotidique à haut débit
/
Nanopores
/
Séquençage du génome entier
Langue:
En
Journal:
Adv Exp Med Biol
Année:
2019
Type de document:
Article
Pays d'affiliation:
Japon
Pays de publication:
États-Unis d'Amérique