CRISPR Screening in Single Cells.
Methods Mol Biol
; 1979: 395-406, 2019.
Article
de En
| MEDLINE
| ID: mdl-31028650
ABSTRACT
The combination of single-cell RNA-seq and CRISPR allows for efficient interrogation of possibly any number of genes, only limited by the sequencing capability. Here we describe the current protocols for CRISPR screening in single cells, from cloning and virus production to generating sequencing data.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Analyse sur cellule unique
/
Systèmes CRISPR-Cas
Type d'étude:
Diagnostic_studies
/
Screening_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2019
Type de document:
Article
Pays d'affiliation:
Suède