Response of lipid biosynthesis in Chlorella pyrenoidosa to intracellular reactive oxygen species level under stress conditions.
Bioresour Technol
; 287: 121414, 2019 Sep.
Article
de En
| MEDLINE
| ID: mdl-31078813
ABSTRACT
An increase in the total lipid content in algal cells under stress conditions is often accompanied by an increase in reactive oxygen species (ROS). However, the link between these two events is unclear. In this study, the regulatory mechanism of ROS formation on lipid accumulation in C. pyrenoidosa was investigated using a Fenton-like reaction. A high Spearman correlation coefficient of 0.901 was obtained between the Hydroxyl radical (OH) level and lipid content. Importantly, the increase in the total lipid content was clearly coupled with a significant increase in the intracellular OH concentration rather than increases in the H2O2 and O2- concentrations. Transcriptome data confirms that most of the differential expression genes (DEGs) involved in fatty acid and glycerolipid biosynthesis were up-regulated by the increased OH under stress conditions. These results reveal that lipid accumulation in algal cells was promoted by OH.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Chlorella
/
Espèces réactives de l'oxygène
/
Lipides
Langue:
En
Journal:
Bioresour Technol
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2019
Type de document:
Article
Pays d'affiliation:
Chine