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Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.
Gwynne, Lauren; Sedgwick, Adam C; Gardiner, Jordan E; Williams, George T; Kim, Gyoungmi; Lowe, John P; Maillard, Jean-Yves; Jenkins, A Toby A; Bull, Steven D; Sessler, Jonathan L; Yoon, Juyoung; James, Tony D.
Affiliation
  • Gwynne L; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Sedgwick AC; Department of Chemistry, University of Texas at Austin, Austin, TX, United States.
  • Gardiner JE; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Williams GT; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Kim G; Department of Chemistry and Nano Science, Ewha Womans University, Seoul, South Korea.
  • Lowe JP; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Maillard JY; Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, United Kingdom.
  • Jenkins ATA; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Bull SD; Department of Chemistry, University of Bath, Bath, United Kingdom.
  • Sessler JL; Department of Chemistry, University of Texas at Austin, Austin, TX, United States.
  • Yoon J; Department of Chemistry and Nano Science, Ewha Womans University, Seoul, South Korea.
  • James TD; Department of Chemistry, University of Bath, Bath, United Kingdom.
Front Chem ; 7: 255, 2019.
Article de En | MEDLINE | ID: mdl-31119120
ABSTRACT
A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Type d'étude: Diagnostic_studies Langue: En Journal: Front Chem Année: 2019 Type de document: Article Pays d'affiliation: Royaume-Uni
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Type d'étude: Diagnostic_studies Langue: En Journal: Front Chem Année: 2019 Type de document: Article Pays d'affiliation: Royaume-Uni