Teburu-Open source 3D printable bioreactor for tissue slices as dynamic three-dimensional cell culture models.
Artif Organs
; 43(10): 1035-1041, 2019 Oct.
Article
de En
| MEDLINE
| ID: mdl-31211867
ABSTRACT
Three-dimensional tissue cultures are important models for the study of cell-cell and cell-matrix interactions, as well as, to investigate tissue repair and reconstruction pathways. Therefore, we designed a reproducible and easy to handle printable bioreactor system (Teburu), that is applicable for different approaches of pathway investigation and targeted tissue repair using human tissue slices as a three-dimensional cell culture model. Here, we definitively describe Teburu as a controlled environment to reseed a 500-µm thick decellularized human liver slice using human mesenchymal stroma cells. During a cultivation period of eight days, Teburu, as a semi-open and low consumption system, was capable to maintain steady pH and oxygenation levels. Its combination with additional modules delivers an applicability for a wide range of tissue engineering approaches under optimal culture conditions.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Bioréacteurs
/
Techniques de culture de tissus
/
Bio-impression
/
Impression tridimensionnelle
Limites:
Humans
Langue:
En
Journal:
Artif Organs
Année:
2019
Type de document:
Article
Pays d'affiliation:
Allemagne