The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop.
Elife
; 82019 07 22.
Article
de En
| MEDLINE
| ID: mdl-31329099
ABSTRACT
Neural progenitors undergo temporal patterning to generate diverse neurons in a chronological order. This process is well-studied in the developing Drosophila brain and conserved in mammals. During larval stages, intermediate neural progenitors (INPs) serially express Dichaete (D), grainyhead (Grh) and eyeless (Ey/Pax6), but how the transitions are regulated is not precisely understood. Here, we developed a method to isolate transcriptomes of INPs in their distinct temporal states to identify a complete set of temporal patterning factors. Our analysis identifies odd-paired (opa), as a key regulator of temporal patterning. Temporal patterning is initiated when the SWI/SNF complex component Osa induces D and its repressor Opa at the same time but with distinct kinetics. Then, high Opa levels repress D to allow Grh transcription and progress to the next temporal state. We propose that Osa and its target genes opa and D form an incoherent feedforward loop (FFL) and a new mechanism allowing the successive expression of temporal identities.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Facteurs de transcription
/
Encéphale
/
Protéines à homéodomaine
/
Régulation de l'expression des gènes au cours du développement
/
Protéines de Drosophila
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Protéines de liaison à l'ADN
/
Drosophila
/
Facteurs de transcription SOX
/
Cellules souches neurales
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Elife
Année:
2019
Type de document:
Article
Pays d'affiliation:
Autriche