[Mechanisms of tanshinone â
¡_A in reducing 4-HNE-induced hepatocyte damage by activating PPARα].
Zhongguo Zhong Yao Za Zhi
; 44(9): 1862-1868, 2019 May.
Article
de Zh
| MEDLINE
| ID: mdl-31342714
ABSTRACT
Tanshinone â
¡_A( Tan â
¡_A),the liposoluble constituents of Salvia miltiorrhiza,can not only ameliorate the lipidic metabolism and decrease the concentration of lipid peroxidation,but also resist oxidation damage,scavenge free radicals and control inflammation,with a protective effect on prognosis after liver function impairment. Therefore,the studies on the exact mechanism of Tan â
¡_A in protecting the liver can provide important theoretical and experimental basis for the prevention and treatment effect of Tan â
¡_A for liver injury. In the present study,the protective effects and mechanism of Tan â
¡_A on 4-hydroxynonenal( 4-HNE)-induced liver injury were investigated in vitro. Normal liver tissues NCTC 1469 cells were used to induce hepatocytes oxidative damages by 4-HNE treatment. The protective effect of Tan â
¡_A on hepatocytes oxidative damages was detected by release amount of lactate dehydrogenase( LDH) analysis and hoechst staining. The protein expression changes of peroxisome proliferator-activated receptor α( PPARα) and peroxisome proliferator response element( PPRE) were analyzed by Western blot analysis in NCTC 1469 cells before and after Tan â
¡_A treatment. The gene expression changes of fatty aldehyde dehydrogenase( FALDH) were analyzed by Real-time polymerase chain reaction( PCR) analysis. The results showed that 4-HNE increased the release amount of LDH,lowered the cell viability of NCTC 1469 cells,and Tan â
¡_A reversed 4-HNE-induced hepatocyte damage. Western blot analysis and RT-PCR analysis results showed that 4-HNE decreased the expression of PPARα and FALDH and increased the expression of 4-HNE. However,the expression of PPARα and FALDH were increased significantly and the expression of 4-HNE was decreased obviously after Tan â
¡_A treatment. This study confirmed that the curative effect of Tan â
¡_A was obvious on hepatocytes damage,and the mechanism may be associated with activating PPARα and FALDH expression as well as scavenging 4-HNE.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Hépatocytes
/
Abiétanes
/
Récepteur PPAR alpha
Limites:
Animals
Langue:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Sujet du journal:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Année:
2019
Type de document:
Article
Pays d'affiliation:
Chine