Thermodynamic profiles of the interactions of suramin, chondroitin sulfate, and pentosan polysulfate with the inhibitory domain of TIMP-3.
FEBS Lett
; 594(1): 94-103, 2020 01.
Article
de En
| MEDLINE
| ID: mdl-31359422
ABSTRACT
Extracellular levels of soluble TIMP-3 are low, reflecting its binding by extracellular matrix (ECM) components including sulfated glycosaminoglycans (SGAGs) and endocytosis via low density lipoprotein receptor-related protein 1. Since TIMP-3 inhibits ECM degradation, the ability of SGAGs to elevate extracellular TIMP-3 is significant for osteoarthritis treatment. Previous studies of such interactions have utilized immobilized TIMP-3 or ligands. Here, we report the thermodynamics of the interactions of the sGAG-binding N-domain of TIMP-3 with chondroitin sulfate, pentosan polysulfate, and suramin in solution using isothermal titration calorimetry. All three interactions are driven by a favorable negative enthalpy change combined with an unfavorable decrease in entropy. The heat capacity changes (ΔCp ) for all of the interactions are zero, indicating an insignificant contribution from hydrophobic interactions.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pentosane polysulfate
/
Suramine
/
Chondroïtines sulfate
/
Inhibiteur tissulaire de métalloprotéinase-3
/
Simulation de docking moléculaire
Limites:
Humans
Langue:
En
Journal:
FEBS Lett
Année:
2020
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique