Analysis of primary cilia in renal tissue and cells.
Methods Cell Biol
; 153: 205-229, 2019.
Article
de En
| MEDLINE
| ID: mdl-31395380
ABSTRACT
Primary cilia are singular, sensory organelles that extend from the plasma membrane of most quiescent mammalian cells. These slender, microtubule-based organelles receive and transduce extracellular cues and regulate signaling pathways. Primary cilia are critical to the development and function of many tissue types, and mutation of ciliary genes causes multi-system disorders, termed ciliopathies. Notably, renal cystic disease is one of the most common clinical features of ciliopathies, highlighting a central role for primary cilia in the kidney. Additionally, acute kidney injury and chronic kidney disease are associated with altered primary cilia lengths on renal epithelial cells, suggesting ciliary dynamics and renal physiology are linked. Here we describe methods to examine primary cilia in kidney tissue and in cultured renal cells. We include immunofluorescence and scanning electron microscopy to determine ciliary localization of proteins and cilia structure. Further, we detail cellular assays to measure cilia assembly and disassembly, which regulate cilia length.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Microscopie électronique à balayage
/
Cils vibratiles
/
Cellules épithéliales
/
Microscopie intravitale
/
Rein
Limites:
Animals
/
Humans
Langue:
En
Journal:
Methods Cell Biol
Année:
2019
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique