Limited predictive value of TFAM in mitochondrial biogenesis.
Mitochondrion
; 49: 156-165, 2019 11.
Article
de En
| MEDLINE
| ID: mdl-31419493
ABSTRACT
Mitochondrial transcription factor A (TFAM) plays an important role in mitochondrial DNA (mtDNA) transcription and replication. In some experimental settings, TFAM expression parallels parameters of mitochondrial biogenesis, which led to a widespread acceptance of TFAM as marker of mitochondrial biogenesis. We modulated TFAM expression in several experimental systems and observed that it fails to consistently parallel mtDNA copy number and expression of mtDNA-encoded polypeptides. We suggest that the use of TFAM as a marker of mitochondrial biogenesis should be avoided outside of systems in which its performance has been carefully validated.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Facteurs de transcription
/
Biogenèse des organelles
/
ADN mitochondrial
/
Protéines mitochondriales
/
Protéines de liaison à l'ADN
/
Mitochondries
Type d'étude:
Prognostic_studies
/
Risk_factors_studies
Limites:
Humans
Langue:
En
Journal:
Mitochondrion
Année:
2019
Type de document:
Article