A theory of centriole duplication based on self-organized spatial pattern formation.
J Cell Biol
; 218(11): 3537-3547, 2019 11 04.
Article
de En
| MEDLINE
| ID: mdl-31451615
ABSTRACT
In each cell cycle, centrioles are duplicated to produce a single copy of each preexisting centriole. At the onset of centriole duplication, the master regulator Polo-like kinase 4 (Plk4) undergoes a dynamic change in its spatial pattern around the preexisting centriole, forming a single duplication site. However, the significance and mechanisms of this pattern transition remain unknown. Using super-resolution imaging, we found that centriolar Plk4 exhibits periodic discrete patterns resembling pearl necklaces, frequently with single prominent foci. Mathematical modeling and simulations incorporating the self-organization properties of Plk4 successfully generated the experimentally observed patterns. We therefore propose that the self-patterning of Plk4 is crucial for the regulation of centriole duplication. These results, defining the mechanisms of self-organized regulation, provide a fundamental principle for understanding centriole duplication.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Centrioles
/
Protein-Serine-Threonine Kinases
Limites:
Humans
Langue:
En
Journal:
J Cell Biol
Année:
2019
Type de document:
Article
Pays d'affiliation:
Japon