Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex.
Cell Rep
; 28(10): 2517-2526.e5, 2019 09 03.
Article
de En
| MEDLINE
| ID: mdl-31484065
ABSTRACT
The endoplasmic reticulum (ER) membrane protein complex (EMC) is a key contributor to biogenesis and membrane integration of transmembrane proteins, but our understanding of its mechanisms and the range of EMC-dependent proteins remains incomplete. Here, we carried out an unbiased mass spectrometry (MS)-based quantitative proteomic analysis comparing membrane proteins in EMC-deficient cells to wild-type (WT) cells and identified 36 EMC-dependent membrane proteins and 171 EMC-independent membrane proteins. Of these, six EMC-dependent and six EMC-independent proteins were further independently validated. We found that a common feature among EMC-dependent proteins is that they contain transmembrane domains (TMDs) with polar and/or charged residues. Mutagenesis studies demonstrate that EMC dependency can be converted in cells by removing or introducing polar and/or charged residues within TMDs. Our studies expand the list of validated EMC-dependent and EMC-independent proteins and suggest that the EMC is involved in handling TMDs with residues challenging for membrane integration.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéomique
/
Complexes multiprotéiques
/
Réticulum endoplasmique
/
Membranes intracellulaires
/
Protéines membranaires
Type d'étude:
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
Cell Rep
Année:
2019
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique