Effect of RhoC silencing on multiple myeloma xenografts and angiogenesis in nude mice.
J Biol Regul Homeost Agents
; 33(5): 1387-1394, 2019.
Article
de En
| MEDLINE
| ID: mdl-31507136
ABSTRACT
In this study, we investigated the expression of RhoC in the multiple myeloma (MM) cell line RPMI- 8226, as well as the effects of silencing RhoC on the growth of tumor xenografts and tumor-induced angiogenesis in nude mice with MM. For this purpose, we transduced RPMI-8226 cells with lentiviral particles overexpressing short hairpin RNAs (shRNA) targeting RhoC. Tumor xenografts were generated by subcutaneously injecting nude mice with RPMI-8226 cells overexpressing control shRNA [negative control (NC) group] or the RhoC shRNA [the experimental (S) group], respectively. RhoC protein and mRNA levels in the tumor xenografts were measured. Nude mice were also subcutaneously inoculated with Matrigel mixed with vascular endothelial growth factor, and CD31 and KI67 levels in the tumor xenografts were measured by immunohistochemistry. Similarly, we assessed tumor xenograft growth and angiogenesis in Matrigel implants in the mice of both groups. We found that RhoC levels, microvessel density, and CD31 labeling index were more reduced in the S group than in the NC group. However, there was no significant difference in the size of tumor xenografts between the 2 groups. The number of new vessels and the neovascular length in the Matrigel implants were significantly lower in the S group than in the NC group. Therefore, we concluded that RhoC expression in myeloma xenografts has important effects on the induction of angiogenesis.
Mots clés
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéine rhoC liant le GTP
/
Myélome multiple
/
Néovascularisation pathologique
Limites:
Animals
Langue:
En
Journal:
J Biol Regul Homeost Agents
Sujet du journal:
BIOLOGIA
/
BIOQUIMICA
Année:
2019
Type de document:
Article
Pays d'affiliation:
Chine