Identification of a novel RIG-I isoform and its truncating variant in Japanese eel, Anguilla japonica.
Fish Shellfish Immunol
; 94: 373-380, 2019 Nov.
Article
de En
| MEDLINE
| ID: mdl-31533080
ABSTRACT
Retinoic acid-inducible gene-I (RIG-I) is a cytoplasmic viral RNA sensor that triggers the production of type I interferons (IFNs) and proinflammatory cytokines during viral infection. RIG-I gene has been identified previously in Japanese eel, Anguilla japonica. In the present study, we have characterized a novel isoform of RIG-I (designated as AjRIG-Ib) and its truncated variant (AjRIG-Ibv). The AjRIG-Ib encodes 940 amino acids (aa) consisting of two N-terminal caspase activation and recruitment domains (CARDs), a DEX(D/H) box RNA helicase domain, and a C-terminal regulatory domain (CTD). The AjRIG-Ibv encodes a protein of 843 aa, that shares similar structural organization with AjRIG-Ib, but lacking CTD. The gene expression analyses showed that AjRIG-Ib and AjRIG-Ibv were detectable in all tissues/organs examined, and AjRIG-Ib was the predominant form. The mRNA level of AjRIG-Ibv was upregulated rapidly at 8â¯h after the Poly IC injection, and the significant increase of AjRIG-Ib was observed at 16 and 24â¯h post-injection (hpi). Laser confocal microscopy showed that AjRIG-Ib and AjRIG-Ibv were both located in cytoplasm. In addition, the overexpression of AjRIG-Ib or AjRIG-Ibv led to the increased activity of IFN promoter in transient transfection assay. Taken together, our results indicated that AjRIG-Ib and AjRIG-Ibv may play cooperative or somewhat complementary roles in coordinating the antiviral response in fish.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Régulation de l'expression des gènes
/
Protéines de poisson
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Maladies des poissons
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Immunité innée
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Anguilla
Type d'étude:
Diagnostic_studies
Limites:
Animals
Langue:
En
Journal:
Fish Shellfish Immunol
Sujet du journal:
BIOLOGIA
/
MEDICINA VETERINARIA
Année:
2019
Type de document:
Article