The effects and mechanisms of GM-CSF on endometrial regeneration.
Cytokine
; 125: 154850, 2020 01.
Article
de En
| MEDLINE
| ID: mdl-31557635
ABSTRACT
BACKGROUND:
Endometrial injury can result in thin endometrium and subfertility. Granulocyte macrophage colony stimulating factor (GM-CSF) contributes to tissue repair, but its role in endometrial regeneration has not been investigated.METHODS:
To determine the effect of GM-CSF on endometrial regeneration, we established a mouse model of thin endometrium by uterine perfusion with 20⯵L 90% ethanol. Thin endometrium in mice was featured by lowered endometrial thickness, decreased expression of Ki67 in glandular cells, and a reduced number of implantation sites. To explore the mechanism of GM-CSF on endometrial regeneration, endometrium was obtained from patients undergoing hysterectomy or hysteroscopy and endometrial biopsy. Effects of GM-CSF on primary cultured human endometrial glandular and stromal cells were examined by the 5-bromo-2'-deoxyuridine (BrdU) proliferation assay and transwell migration assay, followed by exploration of the potential signaling pathway.RESULTS:
GM-CSF intraperitoneal (i.p.) injection significantly increased endometrial thickness, expression of Ki67 in endometrial glandular cells, and the number of implantation sites. GM-CSF significantly promoted proliferation of primary human endometrial glandular cells and migration of stromal cells. GM-CSF activated p-Akt and increased expressions of p70S6K and c-Jun, which were blocked by LY294002.CONCLUSION:
We found that GM-CSF could improve endometrial regeneration, possibly through activating PI3K/Akt signaling pathway.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Régénération
/
Facteur de stimulation des colonies de granulocytes et de macrophages
/
Endomètre
Type d'étude:
Prognostic_studies
Limites:
Adult
/
Animals
/
Female
/
Humans
/
Middle aged
Langue:
En
Journal:
Cytokine
Sujet du journal:
ALERGIA E IMUNOLOGIA
Année:
2020
Type de document:
Article