Evaluation of a handheld device for the measurement of beta-hydroxybutyrate in capillary blood obtained by the puncture of the vulva as well as in venous whole blood in cattle.
Pol J Vet Sci
; 22(3): 557-564, 2019 Sep.
Article
de En
| MEDLINE
| ID: mdl-31560473
ABSTRACT
A negative energy balance is a common condition in high yielding dairy cows causing the production of ketone bodies (KB), including beta-hydroxybutyrate (BHB), defined as subclinical ketosis (SCK) if clinical signs are missing. The aim of the present study was to evaluate a handheld electronic device for the detection of SCK (BHB-concentration > 1.2 mmol/l), in capillary blood and venous whole blood in cows (WellionVet BELUA, MED TRUST Handels GmbH, Marz, Austria) as well as the feasibility of the puncture of the external vulva with a single use lancet. For this purpose, the blood BHB-concentration was tested in 250 venous and capillary blood samples and compared to the results of a certified laboratory. The majority (76.3%) of the animals displayed no signs of discomfort related to the puncture and in 74.2% the procedure was successful on the first attempt. The BHB-concentrations detected in capillary blood showed good agreement with the reference method, both in capillary (correlation coefficient 0.94 (p⟨0.001), Kappa-value 0.89) and venous whole blood (correlation coefficient of 0.95 (p⟨0.001), Kappa-value 0.89). Altogether, 98% of all the samples were correctly classified as SCK or non-SCK by the handheld device in capillary blood (sensitivity 0.96, specificity 0.98) and 97.4% in venous whole blood (sensitivity 0.889, specificity 0.991), respectively. An increase in the correlation by the adaptation of the cut off level could not be achieved for both sampling sites.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Vulve
/
Maladies des bovins
/
Acide 3-hydroxy-butyrique
/
Cétose
Type d'étude:
Diagnostic_studies
Limites:
Animals
Langue:
En
Journal:
Pol J Vet Sci
Sujet du journal:
MEDICINA VETERINARIA
Année:
2019
Type de document:
Article
Pays d'affiliation:
Autriche