Therapeutic targeting of mitochondrial ROS ameliorates murine model of volume overload cardiomyopathy.
J Pharmacol Sci
; 141(1): 56-63, 2019 Sep.
Article
de En
| MEDLINE
| ID: mdl-31611176
ABSTRACT
Concomitant heart failure is associated with poor clinical outcome in dialysis patients. The arteriovenous shunt, created as vascular access for hemodialysis, increases ventricular volume-overload, predisposing patients to developing cardiac dysfunction. The integral function of mitochondrial respiration is critically important for the heart to cope with hemodynamic overload. The involvement, however, of mitochondrial activity or reactive oxygen species (ROS) in the pathogenesis of ventricular-overload-induced heart failure has not been fully elucidated. We herein report that disorganization of mitochondrial respiration increases mitochondrial ROS production in the volume-overloaded heart, leading to ventricular dysfunction. We adopted the murine arteriovenous fistula (AVF) model, which replicates the cardinal features of volume-overload-induced ventricular dysfunction. Enzymatic assays of cardiac mitochondria revealed that the activities of citrate synthase and NADH-quinone reductase (complex â
) were preserved in the AVF heart. In contrast, the activity of NADH oxidase supercomplex was significantly compromised, resulting in elevated ROS production. Importantly, the antioxidant N-acetylcysteine prevented the development of ventricular dilatation and cardiac dysfunction, suggesting a pathogenic role for ROS in dialysis-related cardiomyopathy. A cardioprotective effect was also observed in metformin-treated mice, illuminating its potential use in the management of heart failure complicating diabetic patients on dialysis.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acétylcystéine
/
Espèces réactives de l'oxygène
/
Myocytes cardiaques
/
Thérapie moléculaire ciblée
/
Mitochondries
/
Cardiomyopathies
/
Antioxydants
Type d'étude:
Etiology_studies
Limites:
Animals
Langue:
En
Journal:
J Pharmacol Sci
Sujet du journal:
FARMACOLOGIA
Année:
2019
Type de document:
Article
Pays d'affiliation:
Japon