cDNA Display: A Stable and Simple Genotype-Phenotype Coupling Using a Cell-Free Translation System.
Methods Mol Biol
; 2070: 43-56, 2020.
Article
de En
| MEDLINE
| ID: mdl-31625089
ABSTRACT
A cDNA display method was developed based on the mRNA display method to increase its stability and efficiency for the directed evolution of various kinds of peptides and proteins. In this method, the puromycin-linker is a key molecule to realize smart genotype-phenotype coupling. A recently improved puromycin-linker and its use were explained in detail for the in vitro selection of peptides and proteins using the cDNA display method.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Biosynthèse des protéines
/
ARN messager
/
ADN complémentaire
Limites:
Animals
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2020
Type de document:
Article
Pays d'affiliation:
Japon