Generation of an integration-free iPSC line, ICCSICi006-A, derived from a male Alzheimer's disease patient carrying the PSEN1-G206D mutation.
Stem Cell Res
; 40: 101574, 2019 10.
Article
de En
| MEDLINE
| ID: mdl-31627126
ABSTRACT
The familial form of Alzheimer's disease (FAD), which is caused by mutations in PRESENILIN 1 (PSEN1) and amyloid precursor protein (APP) genes, represents less than 5% of all AD cases and has an early-onset. We report the generation and characterization of an iPSC line derived from a FAD patient carrying the PSEN1-G206D mutation. The iPSC line maintained the original genotype, a normal karyotype, was free from Sendai viral vectors and reprogramming factors (OCT4, SOX2, KLF4 and c-MYC), presented a typical morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lignée cellulaire
/
Préséniline-1
/
Cellules souches pluripotentes induites
/
Maladie d'Alzheimer
Limites:
Adult
/
Humans
/
Male
Langue:
En
Journal:
Stem Cell Res
Année:
2019
Type de document:
Article
Pays d'affiliation:
Espagne