Single cell analysis reveals immune cell-adipocyte crosstalk regulating the transcription of thermogenic adipocytes.
Elife
; 82019 10 23.
Article
de En
| MEDLINE
| ID: mdl-31644425
ABSTRACT
Immune cells are vital constituents of the adipose microenvironment that influence both local and systemic lipid metabolism. Mice lacking IL10 have enhanced thermogenesis, but the roles of specific cell types in the metabolic response to IL10 remain to be defined. We demonstrate here that selective loss of IL10 receptor α in adipocytes recapitulates the beneficial effects of global IL10 deletion, and that local crosstalk between IL10-producing immune cells and adipocytes is a determinant of thermogenesis and systemic energy balance. Single Nuclei Adipocyte RNA-sequencing (SNAP-seq) of subcutaneous adipose tissue defined a metabolically-active mature adipocyte subtype characterized by robust expression of genes involved in thermogenesis whose transcriptome was selectively responsive to IL10Rα deletion. Furthermore, single-cell transcriptomic analysis of adipose stromal populations identified lymphocytes as a key source of IL10 production in response to thermogenic stimuli. These findings implicate adaptive immune cell-adipocyte communication in the maintenance of adipose subtype identity and function.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes
/
Communication cellulaire
/
Régulation de l'expression des gènes
/
Interleukine-10
/
Adipocytes
/
Thermogenèse
/
Sous-unité alpha du récepteur de l'interleukine-10
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Elife
Année:
2019
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique