A fast and convenient solid phase preparation method for releasing N-glycans from glycoproteins using trypsin- and peptide-N-glycosidase F (PNGase F)-impregnated polyacrylamide gels fabricated in a pipette tip.

Yamamoto, Sachio; Ueda, Maki; Kasai, Masataka; Ueda, Yusuke; Kinoshita, Mitsuhiro; Suzuki, Shigeo

Yamamoto, Sachio; Ueda, Maki; Kasai, Masataka; Ueda, Yusuke; Kinoshita, Mitsuhiro; Suzuki, Shigeo.

Affiliation

Yamamoto S; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan. Electronic address: yamamoto@phar.kindai.ac.jp.
Ueda M; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan.
Kasai M; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan.
Ueda Y; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan.
Kinoshita M; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan; Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan.
Suzuki S; Faculty of Pharmaceutical Sciences, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan; Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Japan.

J Pharm Biomed Anal ; 179: 112995, 2020 Feb 05.

Article de En | MEDLINE | ID: mdl-31767225

Sujet(s)
Mots clés

Enzyme reaction; Enzyme-impregnated polyacrylamide gel; Fast release of N-linked glycans; In situ photopolymerization; On-tip method

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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Polyosides / Résines acryliques / Glycoprotéines / Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase Type d'étude: Guideline Langue: En Journal: J Pharm Biomed Anal Année: 2020 Type de document: Article Pays de publication: Royaume-Uni

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