Investigating the protective actions of D-pinitol against arsenic-induced toxicity in PC12 cells and the underlying mechanism.
Environ Toxicol Pharmacol
; 74: 103302, 2020 Feb.
Article
de En
| MEDLINE
| ID: mdl-31786496
ABSTRACT
Arsenic is awfully toxic metalloid responsible for many human diseases all over the world. Contrastingly, D-pinitol is a naturally occurring bioactive dietary compound has antioxidant properties. The objective of this study is to elucidate the protective actions of D-pinitol on arsenic-induced cytotoxicity and explore its controlling role in biomolecular mechanisms in PC12 cells. Obtained results demonstrated that co-exposure of D-pinitol with arsenic increases cell viability, decreases DNA damage and protects PC12 cells from arsenic-induced cytotoxicity by increasing glutathione (GSH) level and glutathione reductase (GR). Protein expression of western blot analysis showed that co-exposure of D-pinitol and arsenic significantly inhibited arsenic-induced autophagy which further suppressed apoptosis through up-regulation of survival factors; mTOR, p-mTOR, Akt, p-Akt, NF-кB, Nrf2, ERK1, GR, Bcl-x and down-regulation of death factors; p53, Bax, cytochrome c, LC3, although arsenic regulated those factors negatively. These results of this study suggested that D-pinitol protects PC12 cells from arsenic-induced cytotoxicity.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Arsenic
/
Agents protecteurs
/
Inositol
Limites:
Animals
/
Humans
Langue:
En
Journal:
Environ Toxicol Pharmacol
Année:
2020
Type de document:
Article
Pays d'affiliation:
Japon