Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects.
Elife
; 92020 01 06.
Article
de En
| MEDLINE
| ID: mdl-31904338
ABSTRACT
Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of the cell the Rod complex and the class-A penicillin-binding proteins (aPBPs). While the Rod complex governs rod-like cell shape, aPBP function is less well understood. aPBPs were previously hypothesized to either work in concert with the Rod complex or to independently repair cell-wall defects. First, we demonstrate through modulation of enzyme levels that aPBPs do not contribute to rod-like cell shape but are required for mechanical stability, supporting their independent activity. By combining measurements of cell-wall stiffness, cell-wall insertion, and PBP1b motion at the single-molecule level, we then present evidence that PBP1b, the major aPBP, contributes to cell-wall integrity by repairing cell wall defects.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Paroi cellulaire
/
Protéines Escherichia coli
/
Protéines de liaison aux pénicillines
/
Escherichia coli
Langue:
En
Journal:
Elife
Année:
2020
Type de document:
Article
Pays d'affiliation:
France