An enzyme-free immunosensor for sensitive determination of procalcitonin using NiFe PBA nanocubes@TB as the sensing matrix.
Anal Chim Acta
; 1097: 169-175, 2020 Feb 08.
Article
de En
| MEDLINE
| ID: mdl-31910957
ABSTRACT
In this work, a label-free electrochemical immunosensor was developed for the detection of procalcitonin (PCT), using toluidine blue functionalized NiFe Prussian-blue analog nanocubes (NiFe PBA nanocubes@TB) as a signal amplifier. NiFe PBA nanocubes was synthesized by a simple and efficient self-templating method in this work. NiFe PBA nanocubes with open-framework construction not only provides a larger specific area to load a mass of antibodies but produces an excellent signal without adding extra reaction reagent. Besides, the electrochemical performance of NiFe PBA nanocubes can be enhanced after functionalized with TB. The developed immunosensor exhibited favorable performance for PCT detection with a linear range from 0.001 to 25â¯ngâ¯mL-1 and a detection limit of 3â¯×â¯10-4â¯ngâ¯mL-1. Moreover, the immunosensor with acceptable reproducibility, selectivity, and stability may provide a new strategy in the clinical detection of PCT.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Chlorure de tolonium
/
Dosage immunologique
/
Nanocomposites
/
Hexacyanoferrates II
/
Procalcitonine
/
Fer
/
Nickel
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
Anal Chim Acta
Année:
2020
Type de document:
Article