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An adipose tissue galectin controls endothelial cell function via preferential recognition of 3-fucosylated glycans.
Maller, Sebastián M; Cagnoni, Alejandro J; Bannoud, Nadia; Sigaut, Lorena; Pérez Sáez, Juan M; Pietrasanta, Lía I; Yang, Ri-Yao; Liu, Fu-Tong; Croci, Diego O; Di Lella, Santiago; Sundblad, Victoria; Rabinovich, Gabriel A; Mariño, Karina V.
Affiliation
  • Maller SM; Laboratorio de Glicómica Funcional y Molecular, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina.
  • Cagnoni AJ; Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina.
  • Bannoud N; Laboratorio de Glicómica Funcional y Molecular, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina.
  • Sigaut L; Laboratorio de Inmunopatología, Facultad de Ciencias Médicas, Instituto de Histología y Embriología de Mendoza (IHEM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Cuyo, Mendoza, Argentina.
  • Pérez Sáez JM; Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Instituto de Física de Buenos Aires (IFIBA-CONICET), Buenos Aires, Argentina.
  • Pietrasanta LI; Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina.
  • Yang RY; Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Instituto de Física de Buenos Aires (IFIBA-CONICET), Buenos Aires, Argentina.
  • Liu FT; Centro de Microscopías Avanzadas (CMA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Croci DO; Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • Di Lella S; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
  • Sundblad V; Laboratorio de Inmunopatología, Facultad de Ciencias Médicas, Instituto de Histología y Embriología de Mendoza (IHEM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Cuyo, Mendoza, Argentina.
  • Rabinovich GA; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.
  • Mariño KV; Instituto de Química Biológica, Ciencias Exactas y Naturales (IQUIBICEN-CONICET), Buenos Aires, Argentina.
FASEB J ; 34(1): 735-753, 2020 01.
Article de En | MEDLINE | ID: mdl-31914594
Upon overnutrition, adipocytes activate a homeostatic program to adjust anabolic pressure. An inflammatory response enables adipose tissue (AT) expansion with concomitant enlargement of its capillary network, and reduces energy storage by increasing insulin resistance. Galectin-12 (Gal-12), an endogenous lectin preferentially expressed in AT, plays a key role in adipocyte differentiation, lipolysis, and glucose homeostasis. Here, we reveal biochemical and biophysical determinants of Gal-12 structure, including its preferential recognition of 3-fucosylated structures, a unique feature among members of the galectin family. Furthermore, we identify a previously unanticipated role for this lectin in the regulation of angiogenesis within AT. Gal-12 showed preferential localization within the inner side of lipid droplets, and its expression was upregulated under hypoxic conditions. Through glycosylation-dependent binding to endothelial cells, Gal-12 promoted in vitro angiogenesis. Moreover, analysis of in vivo AT vasculature showed reduced vascular networks in Gal-12-deficient (Lgals12-/-) compared to wild-type mice, supporting a role for this lectin in AT angiogenesis. In conclusion, this study unveils biochemical, topological, and functional features of a hypoxia-regulated galectin in AT, which modulates endothelial cell function through recognition of 3-fucosylated glycans. Thus, glycosylation-dependent programs may control AT homeostasis by modulating endothelial cell biology with critical implications in metabolic disorders and inflammation.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Adipocytes / Galectines / Cellules endothéliales / Néovascularisation pathologique Limites: Animals Langue: En Journal: FASEB J Sujet du journal: BIOLOGIA / FISIOLOGIA Année: 2020 Type de document: Article Pays d'affiliation: Argentine Pays de publication: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Adipocytes / Galectines / Cellules endothéliales / Néovascularisation pathologique Limites: Animals Langue: En Journal: FASEB J Sujet du journal: BIOLOGIA / FISIOLOGIA Année: 2020 Type de document: Article Pays d'affiliation: Argentine Pays de publication: États-Unis d'Amérique