Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L).
Environ Pollut
; 260: 114002, 2020 May.
Article
de En
| MEDLINE
| ID: mdl-31991361
ABSTRACT
Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ofloxacine
/
Lactuca
Type d'étude:
Risk_factors_studies
Limites:
Humans
Langue:
En
Journal:
Environ Pollut
Sujet du journal:
SAUDE AMBIENTAL
Année:
2020
Type de document:
Article
Pays d'affiliation:
Espagne