Chemogenetic Control of Nanobodies.
Nat Methods
; 17(3): 279-282, 2020 03.
Article
de En
| MEDLINE
| ID: mdl-32066961
ABSTRACT
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Fragments d'immunoglobuline
/
Protéines à fluorescence verte
/
Nanoparticules
/
Anticorps à domaine unique
Limites:
Humans
Langue:
En
Journal:
Nat Methods
Sujet du journal:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Année:
2020
Type de document:
Article
Pays d'affiliation:
Allemagne