Detection of herpes simplex and varicella-zoster virus from skin lesions: comparison of RT-PCR and isothermal amplification for rapid identification.
Diagn Microbiol Infect Dis
; 97(2): 115015, 2020 Jun.
Article
de En
| MEDLINE
| ID: mdl-32088016
We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicella-zoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1â¯+â¯2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana® instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappaâ¯=â¯1). Concordance between Solana HSV1â¯+â¯2/VZV and RT-PCR was 98.3% (kappaâ¯=â¯0.95) for HSV-1 and 99.3% (kappaâ¯=â¯0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Maladies de la peau
/
Techniques d'amplification d'acides nucléiques
/
Réaction de polymérisation en chaine en temps réel
/
Infection à virus varicelle-zona
/
Herpès
Type d'étude:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
Diagn Microbiol Infect Dis
Année:
2020
Type de document:
Article
Pays de publication:
États-Unis d'Amérique