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Detection of herpes simplex and varicella-zoster virus from skin lesions: comparison of RT-PCR and isothermal amplification for rapid identification.
Jevsnik, Monika; Lusa, Lara; Ursic, Tina; Glinsek Biskup, Urska; Petrovec, Miroslav.
Affiliation
  • Jevsnik M; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia. Electronic address: monika.jevsnik@mf.uni-lj.si.
  • Lusa L; Institute of Biostatistics and Medical Informatics, Faculty of Medicine, Vrazov trg 2, 1104 Ljubljana, Slovenia.
  • Ursic T; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.
  • Glinsek Biskup U; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.
  • Petrovec M; Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.
Diagn Microbiol Infect Dis ; 97(2): 115015, 2020 Jun.
Article de En | MEDLINE | ID: mdl-32088016
We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicella-zoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana® instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa = 1). Concordance between Solana HSV1 + 2/VZV and RT-PCR was 98.3% (kappa = 0.95) for HSV-1 and 99.3% (kappa = 0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Maladies de la peau / Techniques d'amplification d'acides nucléiques / Réaction de polymérisation en chaine en temps réel / Infection à virus varicelle-zona / Herpès Type d'étude: Diagnostic_studies / Observational_studies / Prognostic_studies Limites: Humans Langue: En Journal: Diagn Microbiol Infect Dis Année: 2020 Type de document: Article Pays de publication: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Maladies de la peau / Techniques d'amplification d'acides nucléiques / Réaction de polymérisation en chaine en temps réel / Infection à virus varicelle-zona / Herpès Type d'étude: Diagnostic_studies / Observational_studies / Prognostic_studies Limites: Humans Langue: En Journal: Diagn Microbiol Infect Dis Année: 2020 Type de document: Article Pays de publication: États-Unis d'Amérique