A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Cell Rep
; 31(1): 107476, 2020 04 07.
Article
de En
| MEDLINE
| ID: mdl-32268094
ABSTRACT
Recent studies using human pluripotent stem cells (hPSCs) have developed protocols to induce kidney-lineage cells and reconstruct kidney organoids. However, the separate generation of metanephric nephron progenitors (NPs), mesonephric NPs, and ureteric bud (UB) cells, which constitute embryonic kidneys, in in vitro differentiation culture systems has not been fully investigated. Here, we create a culture system in which these mesoderm-like cell types and paraxial and lateral plate mesoderm-like cells are separately generated from hPSCs. We recapitulate nephrogenic niches from separately induced metanephric NP-like and UB-like cells, which are subsequently differentiated into glomeruli, renal tubules, and collecting ducts in vitro and further vascularized in vivo. Our selective differentiation protocols should contribute to understanding the mechanisms underlying human kidney development and disease and also supply cell sources for regenerative therapies.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lignage cellulaire
/
Techniques de culture cellulaire
/
Cellules souches pluripotentes
Type d'étude:
Guideline
Limites:
Humans
Langue:
En
Journal:
Cell Rep
Année:
2020
Type de document:
Article
Pays d'affiliation:
Japon